Maize Starch-Branching Enzyme Isoforms and Amylopectin Structure. In the Absence of Starch-Branching Enzyme IIb, the Further Absence of Starch-Branching Enzyme Ia Leads to Increased Branching

Title: Identification of Multiple Phosphorylation Sites on Maize Endosperm Starch Branching Enzyme IIb, a Key Enzyme in Amylopectin Biosynthesis Running title: Phosphorylation of Starch Branching Enzyme IIb

Starch branching enzyme cDNA from Solanum tuberosum.

Starch the main storage carbohydrate in higher plants Ta& 1. Characterjstjcs of the SBE c-NA from p a t o and is composed of the two polysaccharides, amylopectin (approximately 75%) and amylose (approximately 25%). Amylopectin is a highly branched a-1,4 glucan containing a1,6 branch points, whereas amylose is composed of long, linear a-1,4 glucans, some of which have very few a-1,6 branch point...

Control of starch branching in barley defined through differential RNAi suppression of starch branching enzyme IIa and IIb

The roles of starch branching enzyme (SBE, EC 2.4.1.18) IIa and SBE IIb in defining the structure of amylose and amylopectin in barley (Hordeum vulgare) endosperm were examined. Barley lines with low expression of SBE IIa or SBE IIb, and with the low expression of both isoforms were generated through RNA-mediated silencing technology. These lines enabled the study of the role of each of these i...

A cDNA encoding starch branching enzyme I from maize endosperm.

ADP-Glc pyrophosphorylase (EC 2.7.7.27), starch synthases (EC 2.4.1.21), and SBEs (EC 2.4.1.18) are the key enzymes in the pathway of plant starch biosynthesis. Starch is a polymer of Glc that exists as two fractions, amylose and amylopectin, in maize (Zea mays L.) kernel amyloplasts. The essentially linear polymer amylose contains a-1,4-linked Glc, whereas the branched polymer amylopectin cont...

Glucan affinity of starch synthase IIa determines binding of starch synthase I and starch-branching enzyme IIb to starch granules.

The sugary-2 mutation in maize (Zea mays L.) is a result of the loss of catalytic activity of the endosperm-specific SS (starch synthase) IIa isoform causing major alterations to amylopectin architecture. The present study reports a biochemical and molecular analysis of an allelic variant of the sugary-2 mutation expressing a catalytically inactive form of SSIIa and sheds new light on its centr...